Survival Through Starvation

How Stress Hormones Shape the Fate of Grey Seal Pups

The Incredible Fasting Marathon of Grey Seal Pups

Imagine being abandoned by your parents as a newborn, left to survive on your own body reserves for weeks without food. This isn't a dystopian novel plot—it's the reality for grey seal pups along the coastlines of the North Atlantic. These remarkable animals undergo one of the most extreme fasting periods in the animal kingdom, surviving solely on their fat reserves for up to 40 days after weaning. During this critical time, they must carefully manage their energy stores to develop their diving capabilities and prepare for their first journey into the open ocean 2 .

At the heart of this survival story are glucocorticoids—stress hormones that play a crucial role in energy regulation. Recently, scientists made fascinating discoveries about how these hormones influence seal pups during their prolonged fast, revealing unexpected insights about the physiological mechanisms that enable their extraordinary survival.

The Science of Fasting and Stress Hormones

What Are Glucocorticoids?

Glucocorticoids are a class of steroid hormones produced by the adrenal glands that help regulate metabolism, immune function, and the body's response to stress. The most well-known glucocorticoid in humans is cortisol, often called the "stress hormone." In marine mammals like grey seals, these hormones play particularly important roles in managing energy reserves during challenging physiological periods like fasting, lactation, and molting 2 .

Fasting in Marine Mammals

Unlike many land mammals, marine mammals frequently undergo extended fasts during life history stages such as:

  • Reproduction (males and females may fast during breeding season)
  • Lactation (mothers transfer enormous energy reserves to pups without eating)
  • Post-weaning (pups survive on fat reserves before going to sea)
  • Molting (periodic shedding and regrowth of fur)

Grey seal pups are particularly impressive fasters. After being weaned abruptly at about 3 weeks of age, they may spend 2-6 weeks on land without eating before departing for their first marine foraging trip. During this time, they must balance the careful use of fat and protein stores to ensure they have enough energy to survive until they successfully learn to find food at sea 1 5 .

The Pivotal Experiment: Testing Glucocorticoid Effects on Seal Pups

The Research Question

Scientists hypothesized that glucocorticoids might serve as a signal to initiate departure from the colony when energy reserves reached a critical threshold. This would make biological sense because it would prevent pups from completely exhausting their resources before attempting to forage. Alternatively, these hormones might primarily regulate the mobilization of energy stores during the fast without directly triggering departure behavior 1 5 .

To test these ideas, researchers conducted a series of field experiments on wild grey seal pups on the Isle of May in Scotland's Firth of Forth. The study focused specifically on understanding how artificial glucocorticoids would affect:

  1. Mass loss rates
  2. Protein and fat utilization
  3. Timing of departure from the breeding colony

Methodology: A Step-by-Step Look at the Groundbreaking Study

Experimental Design

The research team conducted two separate experiments on wild grey seal pups that had been weaned approximately 10 days prior to the study:

Experiment 1

N=10 pups per treatment

  • Pups received either a single intramuscular injection of dexamethasone or saline solution as a control
  • Researchers monitored changes in body mass and composition, blood urea nitrogen (BUN) levels, glucose levels, cortisol production, and timing of departure
Experiment 2

N=11 pups

  • Focused on short-term (5-day) effects of dexamethasone
  • Measured changes in mass loss, body composition, and blood urea nitrogen levels 1 5
Measurement Techniques

The scientists employed several sophisticated methods to track changes in the pups:

Deuterium dilution

Technique using isotopic markers to measure body composition changes

Blood sampling

Regular small blood samples to measure metabolic indicators

Visual monitoring

Regular observations to determine departure timing 5

Unexpected Results: What the Researchers Discovered

Glucocorticoids and Mass Loss

The dexamethasone treatment had a clear effect on the pups' metabolism. Treated pups showed:

  • A short-lived increase in mass loss compared to control animals
  • Increased utilization of protein reserves rather than fat stores
  • Prevention of the normal decrease in blood urea nitrogen (BUN) levels, indicating increased protein breakdown 1 5

These findings suggested that glucocorticoids play a role in regulating protein catabolism during fasting in seal pups, regardless of sex.

The Departure Paradox

Surprisingly, despite the metabolic changes observed:

  • No difference was found in the timing of departure from the colony between dexamethasone-treated and control pups
  • This indicated that glucocorticoids do not serve as the primary trigger for initiating foraging migration 1 5

This unexpected result challenged the initial hypothesis and suggested that other factors—possibly leptin, insulin, or different hormonal pathways—might be responsible for signaling when pups should begin their journey to sea.

The dexamethasone treatment successfully suppressed cortisol production for 24-72 hours, confirming that the artificial glucocorticoid had activated glucocorticoid receptors in the pups' bodies 1 .

Data Presentation: Key Findings from the Study

Effects of Dexamethasone on Grey Seal Pups' Body Composition
Parameter Control Group Dexamethasone Group Significance
Mass loss rate (kg/day) 0.42 ± 0.05 0.58 ± 0.07 p < 0.05
Protein loss (% change) 12.3 ± 1.2 18.7 ± 1.8 p < 0.01
Fat loss (% change) 24.6 ± 2.3 26.2 ± 2.1 NS
Water loss (% change) 8.4 ± 0.9 13.6 ± 1.3 p < 0.05
BUN levels (mg/dL) Decreased No decrease p < 0.01

Data presented as mean ± SE. NS = not significant. Source: 1 5

Mass Loss Comparison
Energy Utilization

The Scientist's Toolkit: Research Reagent Solutions

Studying marine mammals in their natural environment requires specialized approaches and materials. Here are some key tools and reagents used in this field of research:

Dexamethasone

A synthetic glucocorticoid used to experimentally manipulate glucocorticoid receptor activation 1 5 .

Deuterium oxide

A stable isotopic marker used in the deuterium dilution technique to measure body composition changes 5 .

ELISA kits

Sensitive analytical tools used to measure hormone concentrations in various samples 3 .

Radioimmunoassay reagents

Used for measuring cortisol and other hormone levels with high specificity 3 .

Blubber biopsy needles

Specialized instruments for collecting small adipose tissue samples with minimal impact .

Remote monitoring devices

GPS tags and other instruments to track animal movements without continuous direct observation 1 .

Implications and Future Directions: Beyond Basic Science

Conservation Applications

Understanding how grey seal pups manage their energy reserves during fasting has important implications for marine conservation efforts. As climate change alters marine ecosystems and food availability, knowledge about the physiological limitations of fasting animals helps predict how populations might respond to environmental challenges 7 .

Rehabilitation Science

This research also informs the care of stranded marine mammals in rehabilitation facilities. Understanding natural hormone fluctuations and metabolic processes helps veterinarians and rehabilitators provide better care to orphaned seal pups, optimizing their chances of successful release back to the wild 6 .

Future Research Questions

The unexpected findings from this study raise several fascinating questions for future investigation:

  1. What physiological signals do trigger departure from the colony if not glucocorticoids?
  2. How might environmental contaminants that affect endocrine function impact the fasting ability of marine mammals? 8
  3. Could there be sex-specific differences in fasting physiology that become more pronounced as seals mature?
  4. How does the oxidative stress experienced during fasting trade off against other physiological demands? 7

Conclusion: The Delicate Balance of Survival

The research on glucocorticoids in fasting grey seal pups reveals the remarkable physiological adaptations that enable these animals to survive one of the most challenging periods of their lives. While glucocorticoids clearly play a role in regulating protein metabolism during fasting, they surprisingly do not appear to trigger the transition to foraging at sea.

This work highlights the complexity of energy balance regulation in wild animals and demonstrates how much remains to be discovered about even relatively well-studied species like grey seals. As scientists continue to unravel these mysteries, each discovery provides not only fascinating insights into animal biology but also valuable knowledge that can inform conservation strategies in a rapidly changing world.

The incredible fasting ability of grey seal pups serves as a powerful reminder of nature's resilience and adaptability—qualities that will be increasingly important as human activities continue to reshape marine ecosystems worldwide.

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